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. 2020 Oct 29;12:10749–10762. doi: 10.2147/CMAR.S263619

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© 2020 Liu et al.

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The effects of PRR34-AS1 downregulation on the malignant features of HCC cells are mediated by the miR-498/FOXO3 axis. (A) RT-qPCR was performed to detect the expression of miR-498 in HuH7 and SNU-182 cells after miR-498 inhibitor or NC inhibitor injection. (B–E) miR-498 inhibitor or NC inhibitor, alongside si-PRR34-AS1, was transfected into HuH7 and SNU-182 cells. Proliferation, apoptosis, migration, and invasion were examined via the CCK-8 assay, flow cytometric analysis, and transwell cell migration and invasion assays (x200 magnification), respectively. (F) Western blotting assessed the efficiency of pcDNA3.1/FOXO3 treatment in HuH7 and SNU-182 cells. (G–J) CCK-8 assay, flow cytometric analysis, and transwell cell migration and invasion assays (x200 magnification) were used to investigate the proliferation, apoptosis, migration, and invasion, respectively, of HuH7 and SNU-182 cells after cotransfection with pcDNA3.1/FOXO3 or pcDNA3.1 and si-PRR34-AS1. *P < 0.05 and **P < 0.01.