Figure 2.

Apatinib induced apoptosis of colorectal cancer cells. CT26 cells (A) and HCT116 cells (B) were treated with indicated doses of apatinib or control (vehicle: 0.1% DMSO) for 24 h. Cells were then analyzed by Annexin V/PI staining. Data shown are representative of three experiments. CT26 cells (C) and HCT116 cells (D) were treated with indicated doses of apatinib for 24h. PARP expression was detected by immunoblotting, and β-Actin was used as a loading control. Results represent three repeated experiments. (E) HCT116 cells were treated with indicated doses of apatinib alone or in combination with Z-VAD-FMK (20 μM) for 24 h. Cell viability was tested by MTT assay. Data are expressed as means ± SEM of three experiments. **P < 0.01 vs control group.