Figure 2: Cytokine activation induces MSC secretion of SPMs.
(A) LXA4 and LXB4 were identified and measured by LC/MS/MS lipidomics [27] in supernatants from human MSCs (3.5×105). MSC were activated with cytomix (IL-1β, TNFα and IFNγ 50ng/ml) for 24 hours prior to collection of supernatents. (B) Representative multiple reaction monitoring LC/MS/MS chromatograms for LXB4 (m/z 351.2 > 163) from untreated and cytomix activated MSCs.