Table 3. Pre and post infusion VCN of cells transduced with LV X-SCID 2^nd gen in the presence or absence of transduction enhancers.

CD34⁺ cells from two normal donors were transduced with LV X-SCID 2^nd gen and transplanted into NBSGW mice (n=5 per group). The mean VCN ± standard deviation (SD) for pre-infused cells, and blood (12 weeks), whole BM, BM hCD34⁺ cells, and spleen (16 weeks) post transplantation are shown. The fold decrease in VCN of indicated tissues [preinfusion VCN/mean VCN of each group] is shown below. VCN, vector copy number, LV, Lentiviral vector; PGE2, prostaglandin E2; BM, bone marrow.

VCN	LV		PGE2		LentiBOOST		LentiBOOST+PGE2
	Donor 1	Donor 2	Donor 1	Donor 2	Donor 1	Donor 2	Donor 1	Donor 2
Preinfusion	2.30	1.06	5.60	2.44	4.63	2.77	6.68	4.42
Blood	0.64 ± 0.03	0.43 ± 0.07	1.53 ± 0.13	0.80 ± 0.09	2.31 ± 0.35	1.77 ± 0.23	2.54 ± 0.6	1.81 ± 0.31
BM	0.86 ± 0.15	0.43 ± 0.04	2.14 ± 0.29	0.91 ± 0.10	3.05 ± 0.79	1.89 ± 0.12	2.83 ± 0.87	2.35 ± 0.91
hCD34+	0.89 ± 0.18	0.45 ± 0.06	2.15 ± 0.42	0.88 ± 0.13	3.12 ± 0.78	1.97 ± 0.39	2.95 ± 1.33	2.43 ± 0.92
Spleen	0.64 ± 0.06	0.37 ± 0.05	1.58 ± 0.18	0.70 ± 0.10	2.36 ± 0.34	1.62 ± 0.17	2.58 ± 0.34	1.96 ± 0.31
Fold decrease versus preinfusion VCN
Blood	3.62	2.49	3.66	3.04	1.79	1.57	2.63	2.44
BM	2.66	2.49	2.62	2.69	1.35	1.46	2.36	1.88
hCD34+	2.59	2.37	2.61	2.76	1.32	1.40	2.27	1.82
Spleen	3.62	2.86	3.55	3.51	1.75	1.71	2.59	2.26