Table 4. Pre and post infusion VCN of cells transduced with LV WASp in the presence or absence of transduction enhancers.

Normal donor CD34⁺ cells were transduced with LV WASp and transplanted into NBSGW mice (n=5 per group). The mean VCN ± SD for pre-infused cells, and blood (12 weeks), whole BM, BM hCD34⁺ cells, and spleen (16 weeks) post transplantation are shown. The fold decrease in VCN of indicated tissues [preinfusion VCN/mean VCN of each group] is shown below. VCN, vector copy number; LV, Lentiviral vector; PGE2, prostaglandin E2; CsH, cyclosporine H; BM, bone marrow.

VCN	LV	PGE2	LentiBOOST	LentiBOOST+PGE2	CsH
Preinfusion	0.46	1.40	0.77	2.16	1.04
Blood	0.15 ± 0.03	0.20 ± 0.05	0.48 ± 0.09	0.42 ± 0.08	0.68 ± 0.2
BM	0.22 ± 0.07	0.22 ± 0.05	0.54 ± 0.10	0.47 ± 0.04	0.75 ± 0.11
hCD34+	0.21 ± 0.12	0.23 ± 0.08	0.53 ± 0.15	0.44 ± 0.09	0.73 ± 0.17
Spleen	0.15 ± 0.03	0.28 ± 0.06	0.48 ± 0.08	0.50 ± 0.04	0.66 ± 0.14
Fold decrease versus preinfusion VCN
Blood	3.11	6.93	1.6	5.19	1.53
BM	2.09	6.42	1.43	4.64	1.39
hCD34+	2.16	6.04	1.46	4.92	1.43
Spleen	3.14	5.07	1.61	4.29	1.58