Skip to main content
. 2020 Nov 2;11:5516. doi: 10.1038/s41467-020-19178-x

Fig. 3. The knock-in open syntaxin mutation enhances evoked neurotransmitter release compared with the wild-type and partially restores the defects of evoked release of snt-1 mutants.

Fig. 3

a Representative EPSCs traces recorded from wild-type N2, open syntaxin, snt-1, and open syntaxin; snt-1 worms in the zxIs6 transgenic background with 10 ms blue light illumination (3.75 mW/mm2). Muscle cells were recorded with a holding potential of −60 mV. be Box-and-whisker plots overlaid with the corresponding data points (squares) of EPSCs amplitude (b), rise time (10–90%) (c), half-width (d), and total charge transfer (e) of wild-type N2 (n = 11), open syntaxin (n = 10), snt-1 (n = 5), and open syntaxin; snt-1 (n = 8) worms, respectively. Two-sample two-sided t-test was performed for the comparison between WT N2 vs. open syntaxin and snt-1 vs. open syntaxin; snt-1 in each parameter. For amplitude (b), N2 vs. open syntaxin: n.s. p = 0.75, snt-1 vs. open syntaxin; snt-1: **p = 0.00. For rise time (c), N2 vs. open syntaxin: n.s. p = 0.10; snt-1 vs. open syntaxin; snt-1: n.s. p = 0.39. For half-width (d), N2 vs. open syntaxin: **p = 0.00, snt-1 vs. open syntaxin; snt-1: *p = 0.03. For total charge transfer (e), N2 vs. open syntaxin: **p = 0.00; snt-1 vs. open syntaxin; snt-1: *p = 0.03. Box-and-whisker plots represent the median (central line), 25th–75th percentile (bounds of the box), and 5th–95th percentile (whiskers).