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. 2020 Nov 2;11:5512. doi: 10.1038/s41467-020-19344-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Cas9 PI domains clustered by their pairwise sequence similarity. Sequences were clustered using CLANS (BLAST option). Lines connect sequences with P value ≤ 1e − 11. Line shading corresponds to P values according to the scale in the top-right corner (light and long lines connect distantly related sequences). For details on how P values are calculated, please see the “Methods” section. Major clusters are shown in bold. Cluster 1 was so named to emphasize that it contains the first experimentally characterized Cas9, Spy. Clusters 2–10 were named beginning from the one with the most members. Different clusters are indicated, and PAM sequences recognized by members of each cluster are highlighted with the associated color. The Cas9 which belongs to the same clade is outlined by a black dashed line. Sequences having known structures are marked red; their PDB code is shown in parentheses.