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. 2020 Nov 2;10:18823. doi: 10.1038/s41598-020-74883-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Evolutionary timescale of Culicidae family. Tree was generated from BEAST using PCGs nucleotide sequences partitioned by gene and codon positions (1st + 2nd and 3rd separately). Blue bars represent the HPD95%. The numbers above and below the bars show the posterior probability and the predicted median dating respectively for each node. Specific words inside the circles represent the nodes discussed in the text. Light blue tip names represent mitogenomes characterized from SRA data. Orange tip names represent sequenced mitogenomes from this study.