(10 Hz, 2 ms, 15 mA, pulse train protocol) as monitored by manometry in anesthetized pigs (n=5–8 each). A) Representative trace of direct transverse colon stimulation; B) Pressure map representation. Channels represent the position of the different manometry probes in the different colonic parts (P: proximal, T: transverse and D: distal). C) Frequency power spectrum in the proximal, transverse and distal colon in response to direct transverse stimulation (cumulative recordings from 4 probes per colonic region in n=5–8 pigs). Direct ES of the transverse colon increased the power of the 1–12 cpm frequencies in the transverse and distal colon with a statistical significant difference in the 2 cpm frequencies in the transverse colon. Data are mean ± SEM, repeated measures two-way ANOVA and Sidak’s post hoc test, * p<0.05 vs baseline in respective groups.