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Specific activity and kinetic parameters of Osc-LOX.
| Substrate | Specific activitya (μmol min−1 mg−1) | Km (μM)a | kcat (min−1)a | kcat/Km (min−1 μM−1)a |
|---|---|---|---|---|
| Arachidonic acid (C20) | 4486.6 ± 45.3 | 68.9 ± 3.5 | 1178.2 ± 16.6 | 17.1 ± 0.6 |
| Eicosapentaenoic acid (C20) | 4941.7 ± 35.2 | 67.0 ± 3.6 | 936.2 ± 27.1 | 14.0 ± 0.4 |
| Docosahexaenoic acid (C22) | 5885.2 ± 61.9 | 63.9 ± 4.8 | 834.5 ± 20.9 | 13.1 ± 0.7 |
aMean values and standard deviations were calculated from three independent experiments.
