Serum IgM (A) and IgG (B) binding to TKO pig red blood cells (RBCs) in human neonates (<30 days of age) (n=24), infants (1–12 months of age, n=40), children (1–6 years of age, n=20), and adults (n=64). (The dotted line represents the lowest limit of detection, indicating no binding.) (A) There is no binding of IgM to TKO pig RBCs during infancy, and only one infant demonstrated any IgG binding. Very few children and adults had any IgM to TKO pig RBCs. (B) Only one infant demonstrated any IgG binding to TKO pig RBCs, and no children or adults demonstrated any binding. (Reprinted with permission from Li et al. 2019.)