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. Author manuscript; available in PMC: 2021 Oct 26.
Published in final edited form as: Dev Cell. 2020 Aug 10;55(2):163–177.e6. doi: 10.1016/j.devcel.2020.07.014

Figure 7. Role of sEVHYP-ILK signaling in mammary gland transformation.

Figure 7.

(A) MCF10A acini in 3D culture seeded in the presence of Ctrl, sEVNORM or sEVHYP (added once at t=0) were analyzed after 21 d for expression of apical polarity marker, GM130, basolateral polarity marker, hDlg, plasma membrane marker, pERM or actin cytoskeleton marker, phalloidin by immunohistochemistry. Representative confocal microscopy images (N=3).

(B) Correlation between ILK expression and overall survival in two independent series of triple-negative breast cancer patients. Kaplan-Meier curves for each dataset are indicated. HR, hazard ratio.

(C) Proposed model for sEVHYP regulation of mitochondrial functions and cell motility in transformation of the normal mammary epithelium via ILK and Akt signaling. See text for details. See also Figures S7.