KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-Ser616-phosphohrylated Drp1 | Cell Signaling | Cat #4494 |
| Mouse monoclonal anti-Drp1 | Cell Signaling | Cat #14647 |
| Rabbit monoclonal anti-MFN1 | Cell Signaling | Cat #14739 |
| Rabbit monoclonal anti-MFN2 | Cell Signaling | Cat #9482 |
| Rabbit monoclonal anti-Ser473-phosphorylated AKT | Cell Signaling | Cat #9271 |
| Rabbit monoclonal anti-Thr308-phosphorylated Akt | Cell Signaling | Cat #4056 |
| Rabbit monoclonal anti-AKT | Cell Signaling | Cat #9272 |
| Rabbit monoclonal anti-EGFR | Cell Signaling | Cat #4267 |
| Rabbit monoclonal anti-MMP9 | Cell Signaling | Cat # 13667 |
| Rabbit monoclonal anti-ILK | Cell Signaling | Cat # 3862 |
| Rabbit monoclonal anti-HIF1α | Cell Signaling | Cat # 36169 |
| Rabbit monoclonal anti-Calnexin | Cell Signaling | Cat # 2679 |
| Rabbit monoclonal anti-VDAC | Cell Signaling | Cat # 4661 |
| Rabbit monoclonal anti-Flottilin1 | Cell Signaling | Cat # 18634 |
| Rabbit monoclonal anti-E-cadherin | Cell Signaling | Cat # 3195 |
| Rabbit monoclonal anti-N-cadherin | Cell Signaling | Cat # 13116 |
| Rabbit monoclonal anti-SNAIL | Cell Signaling | Cat # 3879 |
| Rabbit monoclonal anti-Cleaved-Caspase 3 | Cell Signaling | Cat # 9661 |
| Mouse monoclonal anti-Ki67 | Cell Signaling | Cat # 9449 |
| Rabbit monoclonal anti-pERM | Cell Signaling | Cat # 3726 |
| Rabbit monoclonal anti-Vimentin | Cell Signaling | Cat # 5741 |
| Mouse monoclonal anti-hDlg (SAP97) | Santa Cruz | Cat # sc-9961 |
| Rabbit monoclonal anti-Tsg101 | Protein Tech | Cat # 14497-1-AP |
| Mouse monoclonal anti-CD63 | Abcam | Cat # ab59479 |
| Rabbit monoclonal anti-CD9 | Abcam | Cat # ab223052 |
| Mouse monoclonal anti-β-actin | Sigma | Cat # A5441 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Human Epidermal growth factor | Peprotech | Cat # AF-100-15 |
| Hydrocortisone | Sigma | Cat # H0888 |
| Cholera toxin | Sigma | Cat # C8052 |
| Growth Factor Reduced Matrigel | BD Biosciences | Cat # 354230 |
| Talin-red fluorescent protein (RFP) BacMam | ThermoFisher | Cat # C10612 |
| PKH67 Green Fluorescent Cell Linker | Sigma | Cat # PKH67GL-1KT |
| MitoTracker deep red | ThermoFisher | Cat # M22426 |
| CellLight™ Mitochondria-RFP | ThermoFisher | Cat # C10601 |
| CellROX Green Reagent | ThermoFisher | Cat # C10444 |
| TMRM | ThermoFisher | Cat #M20036 |
| RNAiMAX | ThermoFisher | Cat #13778100 |
| X-tremeGENE HP | Sigma | Cat #366244001 |
| MK-2206 AKT inhibitor | Selleck Chemicals | Cat S1078; CAS: 1032350-13-2 |
| Cpd22 ILK inhibitor | Sigma | Cat #407331–5MG |
| Critical Commercial Assays | ||
| qEV size exclusion column | IZON | qEVoriginal / 70nm |
| Deposited Data | ||
| RNA-seq data | NCBI GEO | Accession number GSE154340 |
| Experimental Models: Cell Lines | ||
| MCF7 | ATCC | ATCC® HTB-22 |
| T47D | ATCC | ATCC® HTB-133™ |
| MCF10A | ATCC | TCC® CRL-10317™ |
| Oligonucleotides | ||
| siRNA targeting HIF1α | Dharmacon | Cat # L-004018 |
| siRNA targeting ILK | Sigma | Cat # PDSIRNA2D |
| siRNA targeting AKT1 | Santa Cruz Biotech | Cat # sc-29195 |
| siRNA targeting AKT2 | Santa Cruz Biotech | Cat # sc-29197 |
| siRNA targeting MFN1 | Santa Cruz Biotech | Cat # sc-270320 |
| siRNA targeting DRP1 | Santa Cruz Biotech | Cat # sc-43732 |
| Recombinant DNA | ||
| 896 pcDNA3 T7 Akt1 | Ramaswamy et al., 1999 | Addgene Cat #9003 |
| 1014 pcDNA3 T7 Akt1 K179M T308A S473A | Ramaswamy et al., 1999 | Addgene Cat #9031 |
| ILK WT | Vaynberg et al., 2018 | N/A |
| L207W ILK mutant | Vaynberg et al., 2018 | N/A |
| Software and Algorithms | ||
| ImageJ | Commercial | https://imagej.nih.gov/ij |
| Prism 8 | Commercial | https://www.graphpad.com/scientificsoftware/prism |
| TrackMate plugin | Commercial | Tinevez et al., 2017 |
| Chemotaxis and Migration Tool v. 2.0 | Ibidi | https://ibidi.com/chemotaxis-analysis/171-chemotaxis-and-migration-tool.html |
| Bowtie 2 | (Langmead and Salzberg, 2012) | N/A |
| RSEM v1.2.12 software | Li and Dewey, 2011 | N/A |
| DESeq2 algorithm | Love et al., 2014 | N/A |
| QIAGEN's Ingenuity® Pathway Analysis software | IPA®, QIAGEN | www.qiagen.com/ingenuity |