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. Author manuscript; available in PMC: 2020 Nov 27.
Published in final edited form as: ACS Nano. 2020 Sep 15;14(10):13268–13278. doi: 10.1021/acsnano.0c05062

Figure 3.

Figure 3.

RGO-PEG promotes efficient antigen delivery to APCs in LNs. (a) Serial PET images of C57BL/6 mice after SC administration of 64Cu-NOTA-Adpgk + CpG and 64Cu-NOTA-RGO(CpG)-PEG-Adpgk. Inguinal and axillary LNs and injection site are indicated by red arrowheads. (b) Time–radioactivity curves of injection site, inguinal and axillary LNs, kidney, intestine, liver, blood, and muscle. (c) 64Cu radioactivity in major organs at 72 h postinjection. (d) Cellular uptake of Adpgk and RGO(CpG)-PEG-Adpgk in CD45+CD11c+ DCs and CD45+F4/80+ macrophages in inguinal LNs at 24 h postinjection. Data represent mean ± SEM from a representative experiment (n = 4) from two independent experiments (a–d). Data were analyzed by one-way ANOVA (b, d) or two-way ANOVA (c) with Tukey’s HSD multiple comparison post hoc test. *P < 0.05, **P < 0.01, ****P < 0.0001.