. Author manuscript; available in PMC: 2021 Oct 1.

Published in final edited form as: Biochem Pharmacol. 2020 Jun 27;180:114127. doi: 10.1016/j.bcp.2020.114127

Table 2.

Summary of intra-day and inter-day accuracy and precision of the LC-MS/MS assay for quantification of Gem, dFdU, and V-Gem at low (0.1 μM), medium (5 μM), and high (50 μM) concentrations in mouse plasma.

		Intra-day (n=3)		Inter-day (n=9)
Analyte	Concentration (μM)	Accuracy (%)	Precision (CV%)	Accuracy (%)	Precision (CV%)
Gem	0.1 / 5 / 50	104 / 108 / 102	3.2 / 2.6 / 6.7	94 / 109 / 103	9.3 / 8.4 / 9.4
dFdU	0.1 / 5 / 50	104 / 108 / 112	4.1 / 3.7 / 4.5	114 / 106 / 102	6.1 / 4.5 / 4.0
V-Gem	0.1 / 5 / 50	107 / 91.1 / 102	2.9 / 12.3 / 6.4	112 / 90 / 108	11.3 / 10.4 / 4.0

Gem, gemcitabine; dFdU, 2’,2’-difluorodeoxyuridine; V-Gem, 5’-l-valyl-gemcitabine.