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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Chem Phys Lipids. 2020 Sep 6;232:104971. doi: 10.1016/j.chemphyslip.2020.104971

Scheme 1.

Scheme 1.

Workflow for lipid metabolic labeling. Derivatized substrate analogs containing clickable groups such as azide, alkyne (not shown) or trans-cyclooctene can be fed to cells to enter biosynthetic pathways and produce tagged products. These products can be derivatized via click reactions including CuAAC, SPAAC, the Staudinger Ligation, and IEDDA to introduce reporter groups such as fluorescent dyes, thereby enabling detection of products by a range of techniques exemplified by fluorescence microscopy, MS and TLC/HPLC.