(A) Representative HPLC chromatogram at 325 nm of lipophilic extracts of 5-days post fertilization zebrafish larvae. (B) Spectral characteristics of extracted retinoids from zebrafish larvae. (C) In vivo two-photon microscopy images of different layers of the zebrafish larval eye. Retinosomes were exclusively detected in the RPE (far right image). 1, 11-cis-retinyl esters (11-RE); 2, all-trans-retinyl esters (at-RE); 3, syn 11-cis-retinal oxime (syn 11-RAL); 4, syn all-trans-retinal (all-RAL); 3’, anti 11-cis-retinal oxime (anti 11-RAL).