Fig 5.

Effect of imatinib on release of cell-free Hb and membrane-derived MPs from healthy RBCs following treatment with the tyrosine phosphatase inhibitor OV. Effect of sodium OV and imatinib on (A) Band 3 tyrosine phosphorylation, (B) release of cell-free Hb, and (C) discharge of membrane-derived MPs from healthy HbAA RBCs. Cells were incubated with the indicated concentrations of imatinib and OV for 4 h with shaking at 1400 rpm prior to analysis. (Error bars are expressed as SEM, * denotes P ≤ 0·05, **denotes P ≤ 0·025, ***denotes P ≤ 0·01 computed from one-way ANOVA) (OV, orthovanadate; Hb, haemoglobin; MPs, microparticles; RBC, red blood cell; SEM, standard error of the mean).