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. Author manuscript; available in PMC: 2021 Nov 7.
Published in final edited form as: Lab Chip. 2020 Sep 18;20(21):3980–3995. doi: 10.1039/d0lc00401d

Figure 2. Analysis of neural rosettes by microplatform seeding onto the NCCIM.

Figure 2.

(a) Neural induction strategies using either custom sized EB intermediates or preformed rosettes in 2D cultures, dissociated and reseeded into the PDMS-fabricated microchamber array (PFMA). Rosette formation in the PFMA from neural-induced EBs generates 3 size categories (1 large rosette, or 3–4 medium rosettes or >5 small rosettes), whereas dissociated NSC rosettes spontaneously form uniformly sized rosette neurospheres in the PFMA (b) Phase images of rosette and rosette neurosphere formation on the PFMA. Scale bar is 100 μm.