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. Author manuscript; available in PMC: 2021 Nov 7.
Published in final edited form as: Lab Chip. 2020 Sep 18;20(21):3980–3995. doi: 10.1039/d0lc00401d

Figure 5. Viability and multipotency of rosettes on the NCCIM.

Figure 5.

(a) Flow diagram from seeding of cells into the PFMA that is combined with the MIST array to generate the assembled NCCIM. Cells were maintained within the NCCIM for 8 hours then evaluated by(b) propidium iodide (PI) staining of EB-derived rosettes and rosette neurospheres to visualize dead cells at hour 0 and hour 8. In (c) neural progenitor marker SOX1 is shown with adherens junction protein ZO-1 that highlights the center of polarized rosette cells. Cells maintain their multipotent state after NCCIM incubation for 8 hours. Scale bar is 50 μm.