Figure 4.

Prediction of redox-active Cys residues in Arabidopsis PGDH1. A, simplified representation of Cys localization of PGDH1 and their conservation in photosynthetic organisms. There are four Cys residues in the A. thaliana PGDH1 mature protein region. The presence or absence of these Cys residues was compared with other PGDH. Other isoforms of A. thaliana PGDH (PGDH2 and PGDH3) and PGDH from other photosynthetic organisms, including Oryza sativa, Nicotiana tabacum, Spinacia oleracea, Populus thrichocarpa, Physcomitrella patens, Marchantia polymorpha, Volvox carteri, Chlamydomonas reinhardtii, Anabaena sp. PCC 7108, Synechococcus elongatus sp. PCC 7942, and Synechocystis sp. PCC 6803, were used for the comparison. The full alignment of PGDH amino acid sequences is shown in Fig. S2. TP, transit peptide. B, three-dimensional structure model of Arabidopsis PGDH1. The model was obtained as a homotetrameric form, but one chain is shown. Four Cys residues are shown by the orange spheres.