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. Author manuscript; available in PMC: 2021 Nov 7.
Published in final edited form as: Nanoscale. 2020 Sep 29;12(41):21172–21187. doi: 10.1039/d0nr04701e

Fig. 1. Flow chart of the overall approach.

Fig. 1.

Human dermal fibroblasts were exposed to different types of well-characterized AuNPs at a low dose (0.1nM) under chronic and non-chronic conditions. After 20 weeks, genome-wide miRNA analysis was performed in cells exposed to AuNPs as well as in controls to assess the long-term changes in miRNA expression levels. Differentially expressed miRNAs were identified. Network-based analysis was also performed to gain a detailed, systems-level view of the miRNA changes. miRNA target genes were bioinfomatically selected and enrichment analysis were carried out to determine biological pathways perturbed by AuNPs exposure. Cellular parameters and mRNA expression data were integrated with miRNA data.