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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: FASEB J. 2020 Sep 12;34(11):14850–14862. doi: 10.1096/fj.201902308RR

Figure 1. Lipidomic analysis of lipoxygenase products during early zebrafish development.

Figure 1.

(A) Schematic depiction of the eicosanoid products of 12-, 5- and 15-Lipoxygenase that are derived from lipid substrates Linoleic Acid (LA), Arachidonic Acid (AA), Docosahexenoic Acid (DHA), and Eicosapentainoic Acid (EPA). (B-D) Two-Way ANOVA was performed for all the lipid products and mRNA comparing amount on each stage (2, 3 and 4 dpf) to 1 dpf. Lipidomic analysis of 12-, 15-, and 5-LOX products in zebrafish embryos aged 1 to 4 days post fertilization (dpf); lipid levels at each age are expressed as nanograms detected normalized to number of embryos (ng/embryos) in each sample. (B) 12-LOX products 12-HETE (triangle) and 12-HEPE (diamond) showed the highest levels, and also the largest increases during development. (C) 15-LOX products 13-HODE, 15-HEPE, 15-HETE, and 17-HDHA showed low levels of expression. (D) 5-LOX products 5-HEPE, 4-HDHA, and 9-HODE were undetectable in embryos; 5-HETE was detected at a low level that remained constant during these stages.