Extended Data Figure 7.

CHEK2 is required for apoptosis of cycling HSPCs, but not for lineage commitment. a, Assessment of IR-induced cell death of cycling HSPCs and myeloid progenitors (CMP, common myeloid progenitor; GMP, granulocyte-monocyte progenitor; MEP, megakaryocyte-erythroid progenitor) following sublethal irradiation, after treatment with CHEK2 inhibitor (n = 3) or dimethylsulfoxide control (n = 3) (two-sided paired t-test). n is the number of biologically independent experiments. Data are mean ± s.e.m. b, Numbers (left) and percent (right) of HSPC colonies formed following CHEK2 inhibition (CHEK2 inhibitor II, Sigma 220486) (n = 4) vs. dimethylsulfoxide (DMSO) control (n = 4). n is the number of biologically independent experiments. Data are mean ± s.e.m. CFU-M, colony forming unit-macrophage; CFU-GM, granulocyte macrophage; CFU-GEMM, granulocyte erythrocyte macrophage megakaryocyte; CFU-G, granulocyte; BFU-E, burst forming unit-erythroid.