Extended Data Figure 8.

Supplementary data for variant-to-function studies at GFI1B locus. a, Map of the lentiviral constructs designed to assess enhancer activity at rs524137. b, Histogram displays GFP mean fluorescence intensity (MFI) of hematopoietic K562 cells infected with Promoter only vs Promoter and Enhancer lentiviral constructs. Compared to mock uninfected control cells, cells infected with the construct carrying both GFI1B promoter and enhancer show greater GFP intensity. c, FACS gating for sorting and identifying the primitive CD34⁺CD45RA⁻CD90⁺CD133⁺EPCR⁺ITGA3⁺ LT-HSC population in day 7 CD34⁺ HSPCs presented in Fig. 4g, h, i. d, Schematic of colony-replating assays using human HSPCs edited with GFI1B coding (CDS) and enhancer guides (ENH). e, Representative western blot measuring GFI1B protein expression 5 days following CRISPR/Cas9 targeting with non-targeting control (NT), or coding regions of GFI1B (g1, g2). LaminB expression used as loading control. LaminB controls was probed on the same blot as the GFI1B. Similar results were obtained in 3 independent experiments. For gel source data, see Supplementary Fig. 3.