Skip to main content
. Author manuscript; available in PMC: 2021 Mar 28.
Published in final edited form as: Nat Methods. 2020 Sep 28;17(11):1139–1146. doi: 10.1038/s41592-020-0953-2

Extended Data Fig. 8. Recording of Ach signal during sleepwake cycle.

Extended Data Fig. 8

a: The schematic illustration and representative recording data of ACh3.0-mut sensor during the sleep-wake cycle in mice.

b: The group data of the fluorescence response of ACh3.0 and ACh3.0-mut sensors in different sleep-wake status (n=5 mice for ACh3.0 and n=6 mice for ACh3.0-mut, p=0.0009 in wake; p=0.049 in NREM; p=0.008 in REM).

c: The representative recording data of ACh3.0-mut sensor during the sleep-wake cycle in mice. Similar results as the representative images were observed for 6 mice.

d: Multiple recording traces of the ACh3.0 sensor during the sleep-wake cycle (from 3 mice, additional to the representative one in Fig. 3g).

All data are shown as mean value +/− SEM, with the error bars or shaded regions indicating SEM.

Two-sides Student’s t test performed in (b); *, p<0.05; **, p<0.01; ***, p<0.001.