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HSQC NMR spectra of 20 kDa 4-arm PEG-azide (A), hpPLP139–151 (B), and 4-arm PLP139–151 (C). hpPLP139–151 (B) shows a unique resonance from the alkyne peak. 4-arm PLP139–151 (C) does not show the resonance from the alkyne peak, indicating that no residual unconjugated PLP139–151 is present in the final product.
