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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: FASEB J. 2020 Sep 4;34(11):14336–14352. doi: 10.1096/fj.202000206R

Figure 3. Knockdown of GATA6-AS1 inhibits the differentiation of cardiomyocytes induced by small molecules.

Figure 3.

The control shRNA or GATA6-AS1 shRNA SCVI273 hiPSCs were induced for cardiomyocyte differentiation using small molecules CHIR990021 and IWR1. (A) qRT-PCR analysis of of GATA6-AS1 and GATA6 expression at differentiation days 0, 2 and 5. The mean fold difference and standard deviation in gene expression were calculated using three replicates. (B) Cell morphology of the control shRNA culture and the GATA6-AS1 shRNA culture during cardiomyocyte differentiation at days 0, 2, 5 and 14. Scale bars = 200 μm. (C) High-content imaging analysis of the differentiated cells detected by NNX2–5 and α-actinin at day 14 in the control shRNA culture and the GATA6-AS1 shRNA culture. Summary of the purity of cardiomyocytes from 12 wells of the control shRNA culture and the GATA6-AS1 shRNA culture. Scale bars = 50 μm. (D) The expression of genes associated with cardiomyocytes was analyzed by qRT-PCR in the control shRNA culture and the GATA6-AS1 shRNA culture at day 14. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001.