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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: FASEB J. 2020 Sep 4;34(11):14336–14352. doi: 10.1096/fj.202000206R

Figure 4. Knockdown of GATA6-AS1 inhibits mesendoderm and mesoderm induction and alters the expression of Wnt signaling genes.

Figure 4.

The expression of cardiac mesodermal and Wnt signaling markers was analyzed by qRT-PCR in the control shRNA and GATA6-AS1 shRNA NKX2–5-eGFP hESC cultures at differentiation days 0, 2, 5, 8 and 14: (A) mesendodermal markers T and MIXL1; (B) mesodermal markers MESP1 and MESP2; (C) Wnt target genes AXIN2 and LEF1; (D) canonical Wnt-signaling genes WNT3A and WNT8A; and (E) non-canonical Wnt signaling genes WNT5A and WNT11. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001.