Figure 1.

Lipid peroxidation is increased in RV failure. (a, b) 4HNE adducts increased with RV failure vs moderate RV hypertrophy, N=2–5/group. (c, d) 2D-gel electrophoresis and western blot were used to detect total protein in representative RV hypertrophy (N=1) and RV failure (N=1) samples. (e, f) 2D-gel electrophoresis and western blot were used to detect the proteins with increased 4HNE-adducts. 27 protein spots where 4HNE signals were higher in RV failure vs. RV hypertrophy were numbered for identification. Mod – moderate; Sev – severe; RV – right ventricle; RVF – right ventricular failure; 4HNE - 4-hydroxynonenal. Data are presented as mean±SEM. **p<0.01.