Table 2.

4HNE modification of metabolic and mitochondrial proteins characterize oxidant stress in RV failure.

Category	Spot Number	4HNE-Modified Protein Name	Accession No.	Fold Change
Metabolism	22	NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial	NDUS3_HUMAN	2.58
	11	NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial	NDUS2_HUMAN	2.55
	12	Elongation factor Tu, mitochondrial	EFTU_HUMAN	2.53
	14	Beta-enolase	ENOB_HUMAN	2.35
	8	Dihydrolipoyl dehydrogenase, mitochondrial	DLDH_HUMAN	2.26
	16	Creatine kinase M-type	KCRM_HUMAN	2.05
	19	Malate dehydrogenase, cytoplasmic	MDHC_HUMAN	2.02
	10	Alpha-enolase	ENOA_HUMAN	1.99
	23	ES1 protein homolog, mitochondrial	ES1_HUMAN	1.88
	13	Fumarate hydratase, mitochondrial	FUMH_HUMAN	1.70
	18	Glyceraldehyde-3-phosphate dehydrogenase	G3P_HUMAN	1.69
	15	Creatine kinase S-type, mitochondrial	KCRS_HUMAN	1.68
	9	Cytochrome b-c1 complex subunit 1, mitochondrial	QCR1_HUMAN	1.66
	5	Aconitate hydratase, mitochondrial	ACON_HUMAN	1.62
	17	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial	NDUAA_HUMAN	1.62
	21	Cytochrome c1, heme protein, mitochondrial	CY1_HUMAN	1.30
Structure	25	Cysteine and glycine-rich protein 3	CSRP3_HUMAN	25.64
	1	Collagen alpha-1(VI) chain	CO6A1_HUMAN	6.43
	7	Desmin	DESM_HUMAN	2.81
	6	Tubulin beta-4B chain	TBB4B_HUMAN	2.25
	2	Myosin Heavy Chain-7	MYH7_HUMAN	1.72
Survival	3	Stress-70 protein, mitochondrial	GRP75_HUMAN	3.48
	24	Superoxide dismutase [Mn], mitochondrial	SODM_HUMAN	1.96
Others	4	Serum albumin	ALBU_HUMAN	3.38
	26	Myoglobin	MYG_HUMAN	3.15
	20	Four and a half LIM domains protein 2	FHL2_HUMAN	1.85
	27	Myoglobin	MYG_HUMAN	1.45

Proteins with increased levels of 4HNE modification detected in RV failure vs. RV hypertrophy by 2D-gel electrophoresis and western blot were identified using mass spectrometry (N=1/group). 27 proteins were increased in RV failure vs. RV hypertrophy with 100% protein score confidence intervals for all proteins (>95% is considered significant). Fold change represents the increase in RV failure vs. RV hypertrophy. 4HNE - 4-Hydroxynonenal.