Fig. 1.

MGF360-12L interferes with IFN-β signaling pathway. 2.5 µg of plasmid encoding MGF360-12L or empty vector (EV) were transfected into HeLa cells. After 12 h, cells were left untreated or transfected with poly (I: C) (5 µg/mL) and incubated for another 12 h. qPCR was performed to measure IFN-β (A), ISG54 (C), ISG56 (D), STING (E), TBK1 (F) mRNA transcription. B 2.5 µg of plasmid encoding MGF360-12L or empty vector (EV) were co-transfected into HeLa cells with the IFN-β promoter-dependent reporter plasmid p125-Luc and with pRL-TK for normalization. After 12 h, cells were left untreated or transfected with poly (I: C) (5 µg/mL) and incubated for another 12 h. Luciferase activity was determined by dual-luciferase assay. Expression data were normalized to the expression of GAPDH. ***P < 0.001; **P < 0.01; *P < 0.05 (compared to cells transfected with empty vector) (n = 3).