Fig. 2.

MGF360-12L inhibits poly (I: C)-induced production of IRF3, AP-1 and NF-κB. Increasing amounts (1.0 µg, 2.5 µg and 5.0 µg) of plasmid encoding MGF360-12L or empty vector (EV) were transfected into HeLa cells. 12 h later, cells were transfected with or without poly (I: C) (5 µg/mL) or TNFα (5 µg/mL). IRF3 (A), AP-1(B), NF-κB (C, E) mRNA level was measured by qPCR. D, F Increasing amounts (1.0 µg, 2.5 µg and 5.0 µg) of plasmid encoding MGF360-12L or empty vector (EV) were co-transfected into HeLa cells with the NF-κB-Luc and with pRL-TK for normalization. After 12 h, cells were left untreated or transfected with poly (I: C) (5 µg/mL) or TNFα (10 µg/mL) and incubated for another 30 min. Luciferase activity was determined by dual-luciferase assay. Expression data were normalized to the expression of GAPDH. ***P < 0.001; **P < 0.01; *P < 0.05 (compared to cells transfected with empty vector) (n = 3). The protein levels of MGF360-12L were determined by Western blotting.