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. 2020 Oct 23;38:101767. doi: 10.1016/j.redox.2020.101767

Fig. 1.

Fig. 1

Mitophagy is induced in RPTC cells during LPS treatment. (A-E). RPTC cells were treated with 100 μg/ml LPS for 0 (Ctrl-control) to 36 h. (A) Representative images of TUNEL staining. (B) Apoptosis. The percentage of cells with typical apoptotic morphology was evaluated. (C) Immunoblots of LC3II/I and GAPDH (internal loading control). (D) Densitometry analysis of LC3II. (E) Immunoblots of TIM23 and TOM20. (F, G) Densitometry analysis of TOM20 and TIM23. (H) Mitophagy in RPTC cells transfected with COX8-EGFP-mCherry. After transfection, the cells were subjected to LPS treatment for 24 h. The red puncta in the bottom panel represent mitochondria in lysosomes with acidic pH. (I) Quantification of the cells with red-only puncta. Data in B, F, G and I are expressed as mean ± SD. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, significantly different from the control group without LPS exposure. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)