Table 2.

Measures of apical and basal dendrites of pyramidal neurons of layers II/III and V/VI expressing GCaMP3 and GCaMP5G, respectively, vs. batch-internal EGFP expressing neurons as control.

Pyramidal cells overexpressing GCaMP3 and GCaMP5G at DIV 14 and DIV 25
	Pyramidal cells in layers II/III		Pyramidal cells in layers V/VI
	ADL (n)	BDL	ADL (n)	BDL
Age, condition (Number of batches)	Segments	Segments	Segments	Segments
(A) DIV 14 Control for GCaMP3 and GCaMP5G (4)	1716 ± 74 (71)	336 ± 15	1468 ± 61 (55)	308 ± 19
	37.1 ± 1.8	8.9 ± 0.5	31.7 ± 1.8	7.8 ± 0.4
GCaMP3 (4)	1072 ± 77 (41)	320 ± 24	1037 ± 69 (50)	300 ± 20
	22.9 ± 1.5	7.5 ± 0.5	21.8 ± 1.9	6.7 ± 0.5
GCaMP5G (3)	1603 ± 69 (42)	315 ± 18	1470 ± 71 (32)	326 ± 21
	36.1 ± 1.7	7.7 ± 0.5	32.8 ± 1.9	8.0 ± 0.5
ANOVA on ranks vs. control	<0.001	0.61	<0.001	0.47
	<0.001	0.094	<0.001	0.022
Mann–Whitney test GCaMP3 vs. control	<0.001		<0.001
	<0.001		<0.001	0.022
(B) DIV 25 Control for GCaMP3 and GCaMP5G (4)	1980 ± 125 (31)	439 ± 28	1876 ± 108 (38)	446 ± 33
	34.9 ± 2.1	8.7 ± 0.5	29.3 ± 1.4	8.3 ± 0.6
GCaMP3 (4)	1449 ± 125 (25)	374 ± 27	1432 ± 140 (23)	337 ± 31
	24.6 ± 2.0	7.3 ± 0.6	24.6 ± 2.3	7.1 ± 0.6
GCaMP5G (3)	1901 ± 126 (31)	394 ± 23	1481 ± 99 (32)	403 ± 42
	32.0 ± 2.0	7.6 ± 0.4	26.7 ± 2.1	7.6 ± 0.6
ANOVA on ranks vs. control	0.015	0.417	0.009	0.003
	<0.001	0.082	0.096	0.040
Mann–Whitney test	0.008		0.025	0.012
GCaMP3 vs. control	<0.001
Mann–Whitney test			0.005
GCaMP5G vs. control

(A) DIV 14. (B) DIV 25. Given are the mean ± SEM, and n, the number of cells analyzed. P values of the Mann–Whitney rank-sum test vs. control performed after the ANOVA on ranks are in italics. ADL, apical dendritic length (μm); BDL, mean (per cell) basal dendritic length (μm); segments, number of dendritic segments. Significant values are bold and p-values are shown in italic.