Table 5. Detection of PIK3CA mutations in three types of samples from benign patients.

Sample	cfDNA				FFPE				gDNA
	Coverage	VarReads	VarFreq		Coverage	VarReads	VarFreq		Coverage	VarReads	VarFreq
1	37,290	7,675	20.60%		2,095	0	0		3,728	1,308	35.12%
2	36,643	11,486	31.37%		705	0	0		1,086	0	0
3	30,621	9,918	32.42%		4,060	1,192	29.42%		1,513	0	0
4	20,275	5,777	28.51%		3,658	0	0		2,164	402	18.61%
5	32,658	9,314	28.55%		2,880	671	23.30%		1,445	252	17.44%
6	37,374	10,626	28.47%		4,405	0	0		1,052	164	15.59%
7	37,490	5,964	15.92%		1,815	0	0		2,687	0	0
8	23,675	7,468	31.56%		2,948	979	33.21%		2,841	0	0
9	31,457	9,374	29.81%		5,377	16,81	31.30%		2,937	538	18.33%
10	38,797	10,842	27.98%		8,679	2,462	28.39%		6,207	0	0

cfDNA, cell-free DNA; FFPE, formalin-fixed and paraffin-embedded.