Fig. 3.

Overexpression of circFNDC3B suppressed cell proliferation, migration and invasion in CRC; the effects were compromised by miR‐937‐5p overexpression. (A) LoVo and SW480 cells were treated with pLCDH‐circFNDC3B alone or co‐transfected with miR‐937‐5p mimics, and the relative expression of circFNDC3B was compared by Q‐PCR. (B) Relative expression of miR‐937‐5p under indicated conditions was determined by Q‐PCR. (C) CCK‐8 assay was performed to determine the proliferation rate in the two cell lines. (D) Clone formation in the two cell lines was evaluated after the indicated treatments. (E) Migration ability of LoVo and SW480 cells in the specific groups was assessed by wound‐healing experiment. Scale bar: 500 μm. (F) Transwell assay was performed to measure the invasion ability in the two cell lines. Scale bar: 200 μm. (G) Differential expression levels of EMT regulators including E‐cadherin, N‐cadherin, MMP‐9, MMP‐2 and slug were determined by western blot. All the experiments were repeated three times. Statistical evaluation was performed using Student’s t test (two‐tailed) between two groups or one‐way ANOVA followed by Tukey’s post hoc test for multiple comparison. Data are expressed as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.