Fig. 6.

Administration of ROCK inhibitor elicits the immune response. Murine tumors after Y‐27632 and/or anti‐PD‐1 antibody treatment were harvested for transcriptomic analysis. (A) The Venn diagram shows differential genes from Y‐27632‐treated and/or anti‐PD‐1 antibody‐treated tumors. Three tumor samples from each group were used (n = 3). (B) The GO pathway analysis of Y‐27632‐treated tumors or control. Three tumor samples from each group were used (n = 3). (C) Heat map showing expression value (z‐score based on cufflink count) of Y‐27632‐treated tumors (n = 2) or control (n = 3). (D) The correlation expression of GZMB with MSN in TCGA dataset was analyzed using the online tool Ulcan. Breast invasive carcinoma samples were analyzed (n = 1097). (E) Survived cell number of MDA‐MB‐231 cells after 3‐day cocultures of Jurkat T cells. Standard error of mean (SEM) from triplicates is shown by vertical bars (n = 3). Statistical significance was assessed by Student’s t‐test. Scale bar: 100 μm. (F) IL‐2 levels in supernatants from 3‐day cocultures of Jurkat T cells and MDA‐MB‐231 cells transfected were detected by the ELISA. *P < 0.05, unpaired two‐tailed t‐test. Standard error of mean (SEM) from triplicates is shown by vertical bars (n = 3). (G) IL‐2 levels in supernatants from 3‐day cocultures of Jurkat T cells and MDA‐MB‐231 cells treated with DMSO or Y‐27632 (20 μm) were detected by the ELISA. Standard error of mean (SEM) from triplicates is shown by vertical bars (n = 3). Statistical significance was assessed by Student’s t‐test.