Fig. 3.

HGSOC pluripotency and self‐renewal. Cells were grown in nonadherent conditions, and spheroids were analyzed on day 7. Spheroid number per 1000 cells (A) and size (B) (PDX9 (n = 5), PDX8 (n = 5), OV8 (n = 5), PDX4 (n = 5), and PDX6 (n = 3); error bars, SEM). PDX14, PDX5, PDX1, and PDX3 did not form spheroids. Pluripotency markers OCT4, NANOG, LIN28A, and HMGA2 were analyzed by RT‐qPCR (C). (D) Western blots of LIN28A and HMGA2 relative to tubulin were quantified on protein level. Full western blots shown in Fig. S5. LIN28A was not detected in PDX3, 5, 9, 8, and OV8.