Table 3.
PDX in vivo model. Nude mice injected orthotopically with PDX. Tumorigenicity refers to percentage of tumors formed at the primary site of injection (ovarian bursa) at euthanasia, maximum 90 days. Metastasis refers to percentage of mice that developed secondary tumors. Numbers of mice orthotopically injected: N = 3, 4, 7, 4, 11, and 6 for PDX3, PDX9, PDX5, PDX8, PDX6, and PDX4 respectively. NSG, NOD scid gamma.
| Sample | Strain | Tumorigenicity (%) | Endpoint reached (%) | Endpoint (days) | Primary tumor (g) | Metastasis (%) | Notes | |
|---|---|---|---|---|---|---|---|---|
|
PDX5 | NSG | 0 | 0 | NA | NA | 0 | |
| PDX9 | Nude | 0 | 0 | NA | NA | 0 | ||
| PDX3 | Nude/NSG | 0 | 0 | NA | NA | 0 | ||
| PDX8 | NSG c | 100 | 100 | 36.3 ± 1.7 | 6.3 ± 0.34 | 100 | a | |
| PDX4 | Nude | 100 | 100 | 44.7 ± 4.7 | 9.24 ± 0.95 | 40 | b | |
| PDX6 | Nude | 90 | 90 | 23.7 ± 2.6 | 5.4 ± 1.2 | 73 | b |
a
Bloody ascites. Peritoneal and liver metastasis. Primary tumor invaded peritoneum and was difficult to excise.
b
Peritoneal metastases.
c
No tumors formed in nude mice.