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. 2020 Sep 5;14(11):2920–2935. doi: 10.1002/1878-0261.12787

Fig. 1.

Fig. 1

AAV‐DNase I transduction of cultured human hepatoma cells leads to DNase I secretion. (A) The schematic construct of pAAV‐ApoEHCR enhancer‐hAAT promoter‐hDNase I (hyperactive)‐WPRE Xinact (AKA, CLS‐014). hAAT promoter, human alpha antitrypsin promoter. (B) DNase I levels were determined at 24–72 h post‐transduction with different AAV vector concentrations in HepG2 cells. Levels of AAV‐DNase I in culture media changes with time and vector concentration. n = 3. (C) Viability of cells as indexed by trypan blue at 5 days after AAV vector exposure to HepG2. AAV‐DNase I did not induce cytotoxicity. n = 3. Data represent mean ± SD. The data were analyzed by two‐way ANOVA. Data represent mean ± SD. ****P < 0.0001.