Fig. 5.

PCK2 and GLUT1 expression are modulated by hypoxia in lung cancer cell lines. Expression of PCK2 protein in H460 (A) and A549 (B) lung cancer cell lines, cultured in high glucose (10 mm) or low glucose (1 mm) media, in normoxia or hypoxia (1% oxygen). Beta‐actin and GAPDH were used as loading controls. Integrated density was normalized to first sample and the loading control. (C and D) GLUT1 (SLC2A1) expression analyzed by quantitative PCR in H460 (C) and A549 (D) cells. Results, displayed as mean +/‐ SEM, were obtained in four (A, B), six (C), or five (D) independent experiments. Group comparisons were performed using Student’s t‐test or one‐group Student’s t‐test as applicable. *P < 0.05, **P < 0.01, ***P < 0.001.