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. 2020 Nov 2;40(11):BSR20201573. doi: 10.1042/BSR20201573

Figure 3. Functional acquisition study of miR-1183 on primary peripheral blood mononuclear cells (PBMCs) and THP-1 cells.

Figure 3

(A and B) Effect of mir-1183 transfection by primary peripheral blood mononuclear cells (PBMCs); (A) under ordinary microscope and (B) under fluorescence microscope. (C and D) Expression of Bcl-2 mRNA in PBMCs and THP-1 cells transfected with mir-1183 mimic. (C) PBMCs Bcl-2 mRNA expression by PCR; (D) THP-1 Bcl-2 mRNA expression by PCR (**P<0.01). (E) Western blot for the detection the expression of Bcl-2 in RHD patients with high expression of mir-1183. (A–C) Three cases of RHD groups; (D–F) Three control groups (scale bar: 50 μm).