Fig. 1.

Calculation of optical wave similarity (OWS) from voltage optical mapping data. A) Fluorescence image of voltage dye loaded guinea pig whole heart. Representative signals are shown from the locations marked in blue and red on the fluorescence image. B) Windowing of optical signals based on signal minima. C) Normalisation and alignment of individual optical action potentials (OAPs) in the recorded optical signal. D) Example calculations of OWS from OAP pairs. E) Constructed map of OWS following analysis steps set out in A-D.