Figure 4.

Clustering of proteins in the Renin-Angiotensin System and major Natriuretic proteins. (A) Hierarchical clustering was used on 15 proteins in the Renin-Angiotensin System. Log₂ fold-change is Lepto/Control. Weighted spectral counts were normalized using the membership function (normalized values are 0 to 1 on the color bar y-axis). Bold indicates differentially abundant proteins (Wilcoxon rank sum using weighted spectral counts, Benjamini–Hochberg adjusted p-value <0.05). CTSG (cathepsin G), DPP3 (dipeptidyl peptidase 3), PREP (prolyl endopeptidase), DNPEP (aspartyl aminopeptidase), CPA2 (carboxypeptidase A2), AGT (angiotensinogen), CTSB (cathepsin B), ATP6AP2 (ATPase H+ transporting accessory protein 2), CTSD (cathepsin D), CPA1 (carboxypeptidase A1), ANPEP (alanyl aminopeptidase, membrane; alternative name, aminopeptidase N), ACE2 (angiotensin I converting enzyme 2), MME (membrane metalloendopeptidase; alternative name, neprilysin), ACE (angiotensin I converting enzyme), ENPEP (glutamyl aminopeptidase; alternative name, aminopeptidase A). (B) Hierarchical clustering was used on five natriuretic peptide proteins. Log₂ fold-change is Lepto/Control. Weighted spectral counts were normalized using the membership function (normalized values are 0 to 1 on the color bar y-axis). Bold indicates differentially abundant proteins (Wilcoxon rank sum using weighted spectral counts, Benjamini–Hochberg adjusted p-value <0.05). GUCA2B (Guanylate cyclase activator 2B), NPPA (Natriuretic peptide A), NPPB (Natriuretic peptide B), NPPC (Natriuretic peptide C), GUCA2A (Guanylate cyclase activator 2A).