TABLE. Oligonucleotide sequence of primers and amplification conditions of the polymerase chain reaction.
| Locus | Sequence(5’-3’) | MgCl2 (mM) | Taq (U) | Primer (µM) | Denaturation | Hybridisation | Extension | Cycles | Product size (bp) |
| pfor | fwd-TGCGGTTTCTGCTCTGTCCAG rev-GTTGCAGCTCTCCGTGTCGAT | 2 | 1 | 1 | 94°C/5 min | 70°C/30 s | 72°C/5 min | 35 | 342 |
| fd | fwd- GACTTCCACCGCATTCGTA rev-AGAGAAGGCAGGCGTAGAGA | 1.5 | 3 | 0.1 | 95°C/5 min | 60°C/30 s | 72°C/7 min | 35 | 251 |
| nr-1 | fwd- AGCGCTGGTCTCTGTTTACC rev-AGTCAACACTTTTCTTCCGGTC | 3 | 3 | 0.1 | 95°C/7 min | 60°C/30 s | 72°C/7 min | 35 | 953 |
| trxr | fwd-CTATAGCCCCGGACGCATTT rev-GTTTCAACATCCCCTCCCCC | 2 | 3 | 0.1 | 95°C/7 min | 62°C/30 s | 70°C/7 min | 35 | 1,273 |
bp: base pairs.