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. 2020 Nov 3;21:269. doi: 10.1186/s13059-020-02186-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — A set of lncRNAs are identified as lnc-eRNAs and are activated through abnormal enhancer transcription. a Analysis of the gene loci of 128 upregulated lncRNAs with H3K27ac, H3K4me1, H3K4me3 and BRD4 enrichment. b Heatmaps of the qPCR assay results showed the expression levels of lnc-eRNAs in MV4-11 cells treated with i-BET151. Eighteen lnc-eRNAs were downregulated, 8 lnc-eRNAs were unchanged, and 3 lnc-eRNAs were not detected in three independent experiments. c The epigenetic environment of SEELA and its genomic relationship with SERINC2. SEELA is located 71.6 kb downstream of SERINC2, and its gene locus is marked by H3K27ac, BRD4 and POL II in two enhancer regions (as shown in schematic diagrams (gray)). The ChIP-qPCR primers were designed to target the gene body of SEELA (black). d DNA agarose gels showing that there were three and two variants of SEELA1 and SEELA2, respectively, according to the results of 5′ and 3′ RACE assays. *Nonspecific band. e The expression of SEELA was significantly upregulated in patients with MLL leukemia (n = 26) compared with MLL-wt (n = 75) patients (MLL-wt, leukemia patients not harboring MLL gene translocation). (Mann-Whitney test; *P < 0.05; ***p < 0.001). The expression level was calculated using the 2^-ΔCT method and was normalized to that of GAPDH. f ChIP-qPCR detection of BRD4, POL II, and MLL fusion protein enrichment at the SEELA1 and SEELA2 gene loci in MV4-11 and RS4;11 cells after treatment with i-BET151. The error bars indicate the ± SEM values (*P < 0.05; **P < 0.01; ***P < 0.001; ns, no significant difference) in three independent experiments