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. 2020 Nov 3;11:23. doi: 10.1186/s43008-020-00045-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Maximum likelihood phylogenetic inference demonstrating the diversity of Archaeorhizomycetes in mid-Sweden pine forest podzol soil based on environmental ribosomal long (41 sequences with ITS and LSU) and short (98 sequences with ITS2) ASVs representing 68 species hypotheses (SH) based on Maximum likelihood solutions in bPTP. Two SHs includes sequences of the described species Archaeorhizomyces borealis and Archaeorhizomyces finlayi. The tree includes the undescribed sister lineage GS31 (Tedersoo, et al. 2017) and four Taphrinomycotina species as outgroup. SHs are cartooned to their stem node to visualize SH represented by more than one ASV. Nine SHs (numbers 1–9) include long read ASVs from the current study and are highlighted in large bold font. Remaining cartooned terminal nodes were represented only by short read itASVs from the current study and are labeled with SH_number or A. borealis. Bootstrap support values over 75 are shown on the branches (calculated from 1000 iterations). Average relative sequence read abundance in soil horizons O, E and B are inserted as tables for two pairs of sister SHs. Bent arrows indicate tested orthogonal contrasts with Pr(>F)-values shown in the middle (See Fig. S7a for corresponding tree with all ASV labels displayed).