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. 2020 Oct 30;19:1533033820947485. doi: 10.1177/1533033820947485

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Effect of sE-cad on lung cancer cell migration and invasion. A549R cells were pretreated with 400 μg/mL DECMA-1 (an sE-cad-blocking antibody) for 4 h and then subjected to Transwell invasion (A) and wound healing (B) assays. A549R cells were pretreated with 0, 800, or 1600 ng/ml rmE-cad for 4 h and then subjected to Transwell invasion (C) and wound healing (D) assays. A549 cells were transfected with the sE-cad overexpression plasmid for 72 h, and western blotting (E) and ELISA (F) were used to detect sE-cad expression. Transwell invasion (G) and wound healing (H) assays were used to detect cell migration and invasion after A549 cells were transfected with the sE-cad overexpression plasmid for 48 h. After A549 cells were pretreated with 0, 800, or 1600 ng/ml rmE-cad for 4 h, Transwell invasion (I) and wound healing (J) assays were used to detect cell migration and invasion. The data are presented as the mean ± SD of 3 independent experiments. *p < 0.05. **p < 0.01. ***p < 0.001.