Figure 6.

Effects of curcumin on the MMP9-sE-cad pathway. (A) Real-time PCR was used to examine E-cadherin cleavage-related gene expression in A549 and A549R cells. A549R cells were exposed to 20 μM curcumin for 48 h, (B) Real-time PCR was used to examine E-cadherin cleavage-related gene expression, (C) Western blot analysis was used to detect MMP9 expression, (D) A549R cells transfected with MMP9 siRNA for 48 h, Western blot analysis was used to detect MMP9 expression, and ELISA was used to detect sE-cad expression. The data are presented as the mean ± SD of 3 independent experiments. **p < 0.01.